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caspase 3 7 activity assay kit  (Elabscience Biotechnology)
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Elabscience Biotechnology caspase 3 7 activity assay kit
HDAC1 regulates the proliferation and apoptosis of GCs. ( A ) Effects TSA treatment at concentration of 0.01, 0.1, 0.25, 0.5, and 1 μM for 24 and 48 h on the viability of porcine GCs. ( B ) Micrographs of GCs treated with 0.1 and 0.25 μM TSA at 24 and 48 h. ( C ) Effects of 0.01, 0.1, 0.25, 0.5, and 1 μM TSA treatment on the expression of HDAC1 for 24 and 48 h. ( D – F ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with TSA. ( G , H ) The proliferation and apoptosis of GCs treated with TSA were assessed using EdU and flow cytometry. ( I ) Effect of TSA on the activity of <t>Caspase</t> 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. ( J ) The interference efficiency of HDAC1 . ( K – M ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with HDAC1 -siRNA. ( N , O ) The proliferation and apoptosis of GCs treated with HDAC1 -siRNA were assessed using EdU and flow cytometry. ( P ) Effect of HDAC1 -siRNA on the activity of Caspase 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. * indicates p < 0.05, ** indicates p < 0.01.
Caspase 3 7 Activity Assay Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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HDAC1 regulates the proliferation and apoptosis of GCs. ( A ) Effects TSA treatment at concentration of 0.01, 0.1, 0.25, 0.5, and 1 μM for 24 and 48 h on the viability of porcine GCs. ( B ) Micrographs of GCs treated with 0.1 and 0.25 μM TSA at 24 and 48 h. ( C ) Effects of 0.01, 0.1, 0.25, 0.5, and 1 μM TSA treatment on the expression of HDAC1 for 24 and 48 h. ( D – F ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with TSA. ( G , H ) The proliferation and apoptosis of GCs treated with TSA were assessed using EdU and flow cytometry. ( I ) Effect of TSA on the activity of Caspase 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. ( J ) The interference efficiency of HDAC1 . ( K – M ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with HDAC1 -siRNA. ( N , O ) The proliferation and apoptosis of GCs treated with HDAC1 -siRNA were assessed using EdU and flow cytometry. ( P ) Effect of HDAC1 -siRNA on the activity of Caspase 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. * indicates p < 0.05, ** indicates p < 0.01.

Journal: Cells

Article Title: HDAC1 -Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p- COX1 Axis

doi: 10.3390/cells12232734

Figure Lengend Snippet: HDAC1 regulates the proliferation and apoptosis of GCs. ( A ) Effects TSA treatment at concentration of 0.01, 0.1, 0.25, 0.5, and 1 μM for 24 and 48 h on the viability of porcine GCs. ( B ) Micrographs of GCs treated with 0.1 and 0.25 μM TSA at 24 and 48 h. ( C ) Effects of 0.01, 0.1, 0.25, 0.5, and 1 μM TSA treatment on the expression of HDAC1 for 24 and 48 h. ( D – F ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with TSA. ( G , H ) The proliferation and apoptosis of GCs treated with TSA were assessed using EdU and flow cytometry. ( I ) Effect of TSA on the activity of Caspase 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. ( J ) The interference efficiency of HDAC1 . ( K – M ) The mRNA and protein levels of cell cycle and cell apoptosis-related genes in GCs treated with HDAC1 -siRNA. ( N , O ) The proliferation and apoptosis of GCs treated with HDAC1 -siRNA were assessed using EdU and flow cytometry. ( P ) Effect of HDAC1 -siRNA on the activity of Caspase 3/7 in porcine GCs was detected using Caspase 3/7 activity assay. * indicates p < 0.05, ** indicates p < 0.01.

Article Snippet: The Caspase 3/7 Activity Assay was also performed to detect the apoptosis of GCs using the Caspase 3/7 Activity Assay Kit (Elabscience, Wuhan, China).

Techniques: Concentration Assay, Expressing, Flow Cytometry, Activity Assay

SFFD as ceRNA for miR-202-3p- COX1 regulates the proliferation and apoptosis of porcine GCs. ( A – D ) Effects of SFFD overexpression and miR-202-3p mimic on the mRNA and protein levels of genes related to cell cycle, E2 secretion, and apoptosis. ( E – H ) Effects of SFFD knockdown and miR-202-3p inhibitor on the mRNA and protein levels of genes related to cell cycle, E2 secretion, and apoptosis. ( I ) Effects of SFFD overexpression and miR-202-3p mimic on the proliferation of GCs. ( J ) Effects of SFFD knockdown and miR-202-3p inhibitor on the proliferation of GCs. ( K ) Effects of SFFD overexpression with miR-202-3p mimic and SFFD knockdown with miR-202-3p inhibitor on the E2 secretion of GCs. ( L , M ) Effects of SFFD overexpression and miR-202-3p mimic on the apoptosis and Caspase 3/7 activity of GCs. ( N , O ) Effects of SFFD knockdown and miR-202-3p inhibitor on the apoptosis and Caspase 3/7 activity of GCs. * indicates p < 0.05, ** indicates p < 0.01.

Journal: Cells

Article Title: HDAC1 -Mediated lncRNA Stimulatory Factor of Follicular Development to Inhibit the Apoptosis of Granulosa Cells and Regulate Sexual Maturity through miR-202-3p- COX1 Axis

doi: 10.3390/cells12232734

Figure Lengend Snippet: SFFD as ceRNA for miR-202-3p- COX1 regulates the proliferation and apoptosis of porcine GCs. ( A – D ) Effects of SFFD overexpression and miR-202-3p mimic on the mRNA and protein levels of genes related to cell cycle, E2 secretion, and apoptosis. ( E – H ) Effects of SFFD knockdown and miR-202-3p inhibitor on the mRNA and protein levels of genes related to cell cycle, E2 secretion, and apoptosis. ( I ) Effects of SFFD overexpression and miR-202-3p mimic on the proliferation of GCs. ( J ) Effects of SFFD knockdown and miR-202-3p inhibitor on the proliferation of GCs. ( K ) Effects of SFFD overexpression with miR-202-3p mimic and SFFD knockdown with miR-202-3p inhibitor on the E2 secretion of GCs. ( L , M ) Effects of SFFD overexpression and miR-202-3p mimic on the apoptosis and Caspase 3/7 activity of GCs. ( N , O ) Effects of SFFD knockdown and miR-202-3p inhibitor on the apoptosis and Caspase 3/7 activity of GCs. * indicates p < 0.05, ** indicates p < 0.01.

Article Snippet: The Caspase 3/7 Activity Assay was also performed to detect the apoptosis of GCs using the Caspase 3/7 Activity Assay Kit (Elabscience, Wuhan, China).

Techniques: Over Expression, Knockdown, Activity Assay